Modified transposases for site-directed insertion of transgenes

Colloms, S. and Renault, S. (2012) Modified transposases for site-directed insertion of transgenes. In: Renault, S. and Duchateau, P. (eds.) Site-Directed Insertion of Transgenes. Series: Topics in current genetics, 23 (23). Springer, pp. 241-265. ISBN 9789400745308 (doi:10.1007/978-94-007-4531-5_9)

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Abstract

Cut and paste DNA transposons are widely used to stably integrate DNA into a wide variety of organisms. They can integrate DNA with high efficiency, provide long lasting expression of inserted transgenes, and avoid some of the safety concerns of viral gene delivery systems. One of the chief disadvantages of transposons for gene therapy and other gene delivery applications is that the site of insertion cannot be chosen. This can lead to poor expression of the integrated gene if it is inserted into a region of heterochromatin, or to undesirable insertional mutagenesis of the host cell if it integrates in an important gene. Three main strategies have been used to direct transposon insertions to specific locations: (1) modifying the transposase by fusing it to a DNA binding domain, (2) tethering the transposase protein to the desired target site, or (3) tethering the transposon DNA to the target site using appropriate fusion proteins. Here we review progress with these strategies in bacteria, fish, insect and mammalian systems.

Item Type:Book Sections
Additional Information:Online ISBN: 9789400745315.
Status:Published
Glasgow Author(s) Enlighten ID:Colloms, Dr Sean
Authors: Colloms, S., and Renault, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Publisher:Springer
ISSN:1610-2096
ISBN:9789400745308

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