A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature

van der Putten, M. A. , MacKenzie, L., Davies, A., Fernandez-Ramos, J., Desai, R., Smith, K. and Harvey, A. (2017) A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature. Physiological Measurement, 38(2), 205. (doi:10.1088/1361-6579/aa5527) (PMID:28001129)

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Abstract

Quantification of blood oxygen saturation (SO2) in vivo is essential for understanding the pathogenesis of diseases in which hypoxia is thought to play a role, including inflammatory disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA). We describe a low-cost multispectral microscope and oximetry technique for calibration-free absolute oximetry of surgically exposed blood vessels in vivo. We imaged the vasculature of the dorsal spinal cord in healthy rats, and varied inspired oxygen (FiO2) in order to evaluate the sensitivity of the imaging system to changes in SO2. The venous SO2 was calculated as 67.8  ±  10.4% (average  ±  standard deviation), increasing to 83.1  ±  11.6% under hyperoxic conditions (100% FiO2) and returning to 67.4  ±  10.9% for a second normoxic period; the venous SO2 was 50.9  ±  15.5% and 29.2  ±  24.6% during subsequent hypoxic states (18% and 15% FiO2 respectively). We discuss the design and performance of our multispectral imaging system, and the future scope for extending this oximetry technique to quantification of hypoxia in inflamed tissue.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fernandez Ramos, Javier and Harvey, Professor Andy and Van Der Putten, Miss Marieke and Davies, Dr Andrew and MACKENZIE, LEWIS
Authors: van der Putten, M. A., MacKenzie, L., Davies, A., Fernandez-Ramos, J., Desai, R., Smith, K., and Harvey, A.
Subjects:?? multispectral imaging ??
?? oximetry ??
?? spinal cord vasculature ??
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Physiological Measurement
Publisher:IOP Publishing
ISSN:0967-3334
ISSN (Online):1361-6579
Published Online:18 January 2017
Copyright Holders:Copyright © 2017 IOP Publishing
First Published:First published in Physiological Measurement 38(2):205
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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