Insulin activates a PD 098059-sensitive kinase that is involved in the regulation of p70S6Kand PHAS-I

Scott, P. H. and Lawrence Jr., J. C. (1997) Insulin activates a PD 098059-sensitive kinase that is involved in the regulation of p70S6Kand PHAS-I. FEBS Letters, 409(2), pp. 171-176. (doi:10.1016/S0014-5793(97)00500-0) (PMID:9202140)

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Abstract

Incubating either Chinese hamster ovary (CHO) cells or 3T3-L1 adipocytes with insulin increased the phosphorylation of the eIF-4E-binding protein, PHAS-I. Insulin also activated p70S6K and the Erk-1 and Erk-2 isoforms of mitogen-activated protein kinase (MAP kinase). However, the concentrations of the hormone needed to activate MAP kinase were 10–100 times higher than those needed to increase PHAS-I phosphorylation and p70S6K activity. Incubating cells with the inhibitor of MAP kinase kinase (MEK) activation, PD 098059, blocked the effects of low concentrations of insulin on PHAS-I and p70S6K. The effects of the inhibitor were overcome by increasing concentrations of insulin. The results indicate that insulin activates a PD 098059-sensitive kinase that is involved in the regulation of both p70S6K and PHAS-I.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Scott, Dr Pamela
Authors: Scott, P. H., and Lawrence Jr., J. C.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:FEBS Letters
Publisher:Elsevier
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:22 August 1997

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