Attempts to image the early inflammatory response during infection with the lymphatic filarial nematode Brugia pahangi in a mouse model

Myburgh, E. , Ritchie, R., Goundry, A. L., O’Neill, K., Marchesi, F. and Devaney, E. (2016) Attempts to image the early inflammatory response during infection with the lymphatic filarial nematode Brugia pahangi in a mouse model. PLoS ONE, 11(12), e0168602. (doi:10.1371/journal.pone.0168602) (PMID:27992545) (PMCID:PMC5161388)

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Abstract

Helminth parasites remain a major constraint upon human health and well-being in many parts of the world. Treatment of these infections relies upon a very small number of therapeutics, most of which were originally developed for use in animal health. A lack of high throughput screening systems, together with limitations of available animal models, has restricted the development of novel chemotherapeutics. This is particularly so for filarial nematodes, which are long-lived parasites with a complex cycle of development. In this paper, we describe attempts to visualise the immune response elicited by filarial parasites in infected mice using a non-invasive bioluminescence imaging reagent, luminol, our aim being to determine whether such a model could be developed to discriminate between live and dead worms for in vivo compound screening. We show that while imaging can detect the immune response elicited by early stages of infection with L3, it was unable to detect the presence of adult worms or, indeed, later stages of infection with L3, despite the presence of worms within the lymphatic system of infected animals. In the future, more specific reagents that detect secreted products of adult worms may be required for developing screens based upon live imaging of infected animals.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Marchesi, Dr Francesco and Devaney, Professor Eileen and Goundry, Miss Amy Louise and Ritchie, Mr Ryan and Myburgh, Dr Elmarie
Authors: Myburgh, E., Ritchie, R., Goundry, A. L., O’Neill, K., Marchesi, F., and Devaney, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2016 Myburgh et al.
First Published:First published in PLoS ONE 11(12):e0168602
Publisher Policy:Reproduced under a creative commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
611411Proteolysis and life cycle progression in LeishmaniaJeremy MottramMedical Research Council (MRC)MR/K019384/1III - PARASITOLOGY