Thomas, F., Burgess, N. C., Thomson, A. R. and Woolfson, D. N. (2016) Controlling the assembly of coiled-coil peptide nanotubes. Angewandte Chemie (International Edition), 55(3), pp. 987-991. (doi: 10.1002/anie.201509304) (PMID:26663438) (PMCID:PMC4744968)
|
Text
132135.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
An ability to control the assembly of peptide nanotubes (PNTs) would provide biomaterials for applications in nanotechnology and synthetic biology. Recently, we presented a modular design for PNTs using α-helical barrels with tunable internal cavities as building blocks. These first-generation designs thicken beyond single PNTs. Herein we describe strategies for controlling this lateral association, and also for the longitudinal assembly. We show that PNT thickening is pH sensitive, and can be reversed under acidic conditions. Based on this, repulsive charge interactions are engineered into the building blocks leading to the assembly of single PNTs at neutral pH. The building blocks are modified further to produce covalently linked PNTs via native chemical ligation, rendering ca. 100 nm-long nanotubes. Finally, we show that small molecules can be sequestered within the interior lumens of single PNTs.
| Item Type: | Articles |
|---|---|
| Additional Information: | F.T. and D.N.W. are grateful for financial support from the Leverhulme Trust (RPG-2012-536). N.C.B. was funded by the EPSRC Bristol Centre for Functional Nanomaterials Centre for Doctoral Training (EP/G036780/1). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Thomson, Dr Drew |
| Authors: | Thomas, F., Burgess, N. C., Thomson, A. R., and Woolfson, D. N. |
| College/School: | College of Science and Engineering > School of Chemistry |
| Journal Name: | Angewandte Chemie (International Edition) |
| Publisher: | Wiley |
| ISSN: | 1433-7851 |
| ISSN (Online): | 1521-3773 |
| Published Online: | 14 December 2015 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Angewandte Chemie (International Edition) 55(3): 987-991 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details