Controlling the assembly of coiled-coil peptide nanotubes

Thomas, F., Burgess, N. C., Thomson, A. R. and Woolfson, D. N. (2016) Controlling the assembly of coiled-coil peptide nanotubes. Angewandte Chemie (International Edition), 55(3), pp. 987-991. (doi:10.1002/anie.201509304) (PMID:26663438) (PMCID:PMC4744968)

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Abstract

An ability to control the assembly of peptide nanotubes (PNTs) would provide biomaterials for applications in nanotechnology and synthetic biology. Recently, we presented a modular design for PNTs using α-helical barrels with tunable internal cavities as building blocks. These first-generation designs thicken beyond single PNTs. Herein we describe strategies for controlling this lateral association, and also for the longitudinal assembly. We show that PNT thickening is pH sensitive, and can be reversed under acidic conditions. Based on this, repulsive charge interactions are engineered into the building blocks leading to the assembly of single PNTs at neutral pH. The building blocks are modified further to produce covalently linked PNTs via native chemical ligation, rendering ca. 100 nm-long nanotubes. Finally, we show that small molecules can be sequestered within the interior lumens of single PNTs.

Item Type:Articles
Additional Information:F.T. and D.N.W. are grateful for financial support from the Leverhulme Trust (RPG-2012-536). N.C.B. was funded by the EPSRC Bristol Centre for Functional Nanomaterials Centre for Doctoral Training (EP/G036780/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Thomson, Dr Drew
Authors: Thomas, F., Burgess, N. C., Thomson, A. R., and Woolfson, D. N.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Angewandte Chemie (International Edition)
Publisher:Wiley
ISSN:1433-7851
ISSN (Online):1521-3773
Published Online:14 December 2015
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Angewandte Chemie (International Edition) 55(3): 987-991
Publisher Policy:Reproduced under a Creative Commons License

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