Functionalized α-helical peptide hydrogels for neural tissue engineering

Mehrban, N. et al. (2015) Functionalized α-helical peptide hydrogels for neural tissue engineering. ACS Biomaterials Science and Engineering, 1(6), pp. 431-439. (doi:10.1021/acsbiomaterials.5b00051) (PMID:26240838) (PMCID:PMC4517957)

[img]
Preview
Text
132132.pdf - Published Version

4MB

Abstract

Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.

Item Type:Articles
Additional Information:This work was supported by the Biotechnology and Biological Sciences Research Council (H01716X, D.N.W. and M.A.B.); the European Research Council (StG243261, BS; and ADG340764, D.N.W.); the Royal Society (UF051616, B.S.); the Medical Research Council (G1100623, A.D.R.); and the Engineering and Physical Sciences Research Council (Bristol Chemical Synthesis Centre for Doctoral Training, EP/G036764/1, K.L.H.). D.N.W. holds a Royal Society Wolfson Research Merit Award.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Thomson, Dr Drew
Authors: Mehrban, N., Zhu, B., Tamagnini, F., Young, F. I., Wasmuth, A., Hudson, K. L., Thomson, A. R., Birchall, M. A., Randall, A. D., Song, B., and Woolfson, D. N.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:ACS Biomaterials Science and Engineering
Publisher:American Chemical Society
ISSN:2373-9878
ISSN (Online):2373-9878
Published Online:28 April 2015
Copyright Holders:Copyright © 2015 American Chemical Society
First Published:First published in ACS Biomaterials Science and Engineering 1(6): 431-439
Publisher Policy:Reproduced in accordance with the publisher copyright policy

University Staff: Request a correction | Enlighten Editors: Update this record