Designed α-helical tectons for constructing multicomponent synthetic biological systems

Bromley, E. H.C., Sessions, R. B., Thomson, A. R. and Woolfson, D. N. (2009) Designed α-helical tectons for constructing multicomponent synthetic biological systems. Journal of the American Chemical Society, 131(3), pp. 928-930. (doi:10.1021/ja804231a) (PMID:19115943)

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Abstract

One possible route to develop new synthetic-biological systems is to assemble discrete nanoscale objects from programmed peptide-based building blocks. We describe an algorithm to design such blocks based on the coiled-coil protein-folding motif. The success of the algorithm is demonstrated by the production of six peptides that form three target parallel, blunted-ended heterodimers in preference to any of the other promiscuous pairings and alternate configurations, for example, homodimers, sticky-ended assemblies, and antiparallel arrangements. The peptides were linked to promote the assembly of larger, defined nanoscale rods, thus demonstrating that targeted peptide−peptide interactions can be specified in complex mixtures.

Item Type:Articles
Additional Information:The authors thank the EPSRC (GR/T09224/01) and HFSP (RPG31/2007) for funding.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Thomson, Dr Drew
Authors: Bromley, E. H.C., Sessions, R. B., Thomson, A. R., and Woolfson, D. N.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Journal of the American Chemical Society
Publisher:American Chemical Society
ISSN:0002-7863
ISSN (Online):1520-5126
Published Online:30 December 2008

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