Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model

Townsend, E. M., Sherry, L., Rajendran, R., Hansom, D., Butcher, J., Mackay, W. G., Williams, C. and Ramage, G. (2016) Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. Biofouling, 32(10), pp. 1259-1270. (doi:10.1080/08927014.2016.1252337) (PMID:27841027)

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Abstract

Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and Rajendran, Dr Ranjith and Sherry, Dr Leighann and Townsend, Miss Eleanor
Authors: Townsend, E. M., Sherry, L., Rajendran, R., Hansom, D., Butcher, J., Mackay, W. G., Williams, C., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Biofouling
Publisher:Taylor & Francis
ISSN:0892-7014
ISSN (Online):1029-2454
Published Online:14 November 2016
Copyright Holders:Copyright © 2016 Taylor and Francis
First Published:First published in Biofouling 32(10): 1259-1270
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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