Simple methods for preparing recombinant adenoviruses for high-efficiency transduction of vascular cells

Nicklin, S. A. and Baker, A. H. (1999) Simple methods for preparing recombinant adenoviruses for high-efficiency transduction of vascular cells. In: Baker, A. H. (ed.) Vascular Disease: Molecular Biology and Gene Transfer Protocols. Series: Methods in molecular medicine (30). Humana Press: Totowa, NJ, pp. 271-284. ISBN 9780896037311 (doi: 10.1385/1-59259-247-3:271)

Abstract

Adenoviruses are icosahedral viruses 70–90 nm in diameter with a double-stranded, linear DNA genome of approx 36 kb. They are widely utilized in gene transfer protocols owing to their relative ease of genetic manipulation, ability to grow to high titres (109–1012 plaque forming units [pfu]/mL), and their ability to infect both dividing and nondividing cells efficiently. The latter reason makes them extremely suitable for investigations in vascular systems where the low proliferative indices of cells is a limiting factor for retroviral gene transfer.

• Item Type: Book Sections
• Status: Published
• Glasgow Author(s) Enlighten ID: Nicklin, Professor Stuart and Baker, Professor Andrew
• Authors: Nicklin, S. A., and Baker, A. H.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Publisher: Humana Press
• ISBN: 9780896037311