Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay

Hu, C. , Zeimpekis, I., Sun, K., Anderson, S., Ashburn, P. and Morgan, H. (2016) Low-cost nanoribbon sensors for protein analysis in human serum using a miniature bead-based enzyme-linked immunosorbent assay. Analytical Chemistry, 88(9), pp. 4872-4878. (doi: 10.1021/acs.analchem.6b00702) (PMID:27035411)

Abstract

We describe a low cost thin-film transistor (TFT) nanoribbon sensor for detection of the inflammatory biomarker C-reactive protein (CRP) in human serum via a miniature bead-based enzyme-linked immunosorbent assay (ELISA). The TFT nanoribbon sensor measures the reaction products from the ELISA via pH changes. The bead-based ELISA decouples the protein functionalization steps from the sensor surface, increasing the signal and simplifying the assay. The ability to directly sense proteins in human serum in this way overcomes the Debye length limitation associated with nanowire and nanoribbon biosensors. Compared to classically fabricated nanowires, the TFT nanoribbon sensors are simple, extremely easy to fabricate, and should therefore be much cheaper to manufacture. TFT nanoribbon sensors, configured to measure pH, were used for quantitative detection of CRP spiked into human serum at concentrations as low as 0.2 ng/mL, which is 10 000 times lower than needed for diagnostic purposes, providing the potential for applications that require very high sensitivity.

• Item Type: Articles
• Additional Information: The authors would like to acknowledge the Technology Strategy Board (TSB) and the Engineering and Physical Sciences Research Council (EPSRC, Grant EP/K502327/1) for funding this work.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Morgan, Hywel and Hu, Dr Chunxiao
• Authors: Hu, C., Zeimpekis, I., Sun, K., Anderson, S., Ashburn, P., and Morgan, H.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Analytical Chemistry
• Publisher: American Chemical Society
• ISSN: 0003-2700
• ISSN (Online): 1520-6882
• Published Online: 01 April 2016