Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3)

Braceland, M., Tinsley, J.., Cockerill, D., Bickerdike, R., McLoughlin, M.F. and Eckersall, P.D. (2017) Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic Salmon, Salmo salar, infected with Salmonid Alpha-Virus (SAV3). Journal of Fish Diseases, 40(8), pp. 1077-1087. (doi: 10.1111/jfd.12587) (PMID:27905123) (PMCID:PMC5516131)

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Abstract

While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on-farm qualitative test and an in-laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ340). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ340 in infected fish rose from undetectable to a maximum at 6 weeks post-infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor David and Braceland, Mr Mark
Authors: Braceland, M., Tinsley, J.., Cockerill, D., Bickerdike, R., McLoughlin, M.F., and Eckersall, P.D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Fish Diseases
Publisher:Wiley
ISSN:0140-7775
ISSN (Online):1365-2761
Published Online:30 November 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Journal of Fish Diseases 40(8): 1077-1087
Publisher Policy:Reproduced under a creative commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
530451Genotyping and Phenotyping Biomarkers of the Pathological Response to Pancreas Disease in the Atlantic Salmon.Peter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/I532837/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED
683931Acute phase protein as biomarkers of disease in livestock and aquaculturePeter EckersallBiotechnology and Biological Sciences Research Council (BBSRC)BB/M022021/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED