Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates

Tsimbouri, P.M. , Fisher, L., Holloway, N., Sjostrom, T., Meek, R.M.D., Su, B. and Dalby, M.J. (2016) Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates. Scientific Reports, 6, 36857. (doi:10.1038/srep36857) (PMID:27857168) (PMCID:PMC5114696)

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Abstract

Nanotopographical cues on Ti have been shown to elicit different cell responses such as cell differentiation and selective growth. Bone remodelling is a constant process requiring specific cues for optimal bone growth and implant fixation. Moreover, biofilm formation and the resulting infection on surgical implants is a major issue. Our aim is to identify nanopatterns on Ti surfaces that would be optimal for both bone remodelling and for reducing risk of bacterial infection. Primary human osteoblast/osteoclast co-cultures were seeded onto Ti substrates with TiO2 nanowires grown under alkaline conditions at 240 °C for different times (2, 2.5 or 3 h). Cell growth and behaviour was assessed by scanning electron microscopy (SEM), immunofluorescence microscopy, histochemistry and quantitative RT-PCR methods. Bacterial colonisation of the nanowire surfaces was also assessed by confocal microscopy and SEM. From the three surfaces tested the 2 h nanowire surface supported osteoblast and to a lesser extent osteoclast growth and differentiation. At the same time bacterial viability was reduced. Hence the 2 h surface provided optimal bone remodeling in vitro conditions while reducing infection risk, making it a favourable candidate for future implant surfaces.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tsimbouri, Dr Penelope and Dalby, Professor Matthew
Authors: Tsimbouri, P.M., Fisher, L., Holloway, N., Sjostrom, T., Meek, R.M.D., Su, B., and Dalby, M.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Reports 6: 36857
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.261

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
615571Multiscale topographical modulation of cells and bacteria for next generation orthopaedic implantsMatthew DalbyEngineering & Physical Sciences Research Council (EPSRC)EP/K034898/1RI MOLECULAR CELL & SYSTEMS BIOLOGY