Purification and characterization of an extracellular lipase from a novel strain Penicillium sp. DS-39 (DSM 23773)

Dheeman, D. S., Antony-Babu, S., Frías, J. M. and Henehan, G. T.M. (2011) Purification and characterization of an extracellular lipase from a novel strain Penicillium sp. DS-39 (DSM 23773). Journal of Molecular Catalysis B: Enzymatic, 72(3-4), pp. 256-262. (doi: 10.1016/j.molcatb.2011.06.013)

Abstract

A newly isolated fungal strain, Penicillium sp. DS-39 (DSM 23773), was found to produce an inducible extracellular lipase when grown in a medium containing 1.0% (v/v) olive oil. Maximum lipase activity was obtained after 120 h of incubation at 28 °C. The lipase was purified 129-fold with a final specific activity of 308.73 IU/mg. The molecular weight of the homogeneous lipase was 43 kDa as determined by SDS-PAGE. It was optimally active at pH 5.5 and 45 °C. The lipase was most active on triolein and exhibited a broad substrate range with a preference for triacylglycerols containing long chain unsaturated fatty acids. It showed no regio-specificity for the ester bond in triolein. It was activated by Ca2+ and Mn2+, while significant inhibition was observed with Hg2+ and Zn2+. The lipase showed significant stability and activation in the presence of organic solvents with log P ≥ 2.0. These features render Penicillium sp. DS-39 lipase (PEL) a potential biocatalyst for applications such as biodiesel production, enzymatic restructuring, by interesterification of different oils and fats, and biodegradation of oil spills in the environment.

• Item Type: Articles (Other)
• Additional Information: This research work was financially supported by DIT ABBEST Research Scholarship to DSD (PB 03557/2007).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Dheeman, Dr Dharmendra
• Authors: Dheeman, D. S., Antony-Babu, S., Frías, J. M., and Henehan, G. T.M.
• Subjects: Q Science > Q Science (General)
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Molecular Catalysis B: Enzymatic
• Publisher: Elsevier
• ISSN: 1381-1177
• Published Online: 28 June 2011