A 3-D cell culture system to study epithelia functions using microcarriers

Jakob, P. H., Kehrer, J., Flood, P., Wiegel, C., Haselmann, U., Meissner, M. , Stelzer, E. H.K. and Reynaud, E. G. (2016) A 3-D cell culture system to study epithelia functions using microcarriers. Cytotechnology, 68(5), pp. 1813-1825. (doi:10.1007/s10616-015-9935-0) (PMID:26847791)

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Abstract

In vitro cell culture models used to study epithelia and epithelial diseases would benefit from the recognition that organs and tissues function in a three-dimensional (3D) environment. This context is necessary for the development of cultures that more realistically resemble in vivo tissues/organs. Our aim was to establish and characterize biologically meaningful 3D models of epithelium. We engineered 3D epithelia cultures using a kidney epithelia cell line (MDCK) and spherical polymer scaffolds. These kidney epithelia were characterized by live microscopy, immunohistochemistry and transmission electron microscopy. Strikingly, the epithelial cells displayed increased physiological relevance; they were extensively polarized and developed a more differentiated phenotype. Using such a growth system allows for direct transmission and fluorescence imaging with few restrictions using wide-field, confocal and Light Sheet Fluorescence Microscopy. We also assessed the wider relevance of this 3D culturing technique with several epithelial cell lines. Finally, we established that these 3D micro-tissues can be used for infection as well as biochemical assays and to study important cellular processes such as epithelial mesenchymal transmission. This new biomimetic model could provide a broadly applicable 3D culture system to study epithelia and epithelia related disorders.

Item Type:Articles
Additional Information:This work was funded by BMBF Grant ‘‘QuantPro’’, Number 0313831D. EGR acknowledges support from the SFI under the Stokes Fellowship Programme.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus
Authors: Jakob, P. H., Kehrer, J., Flood, P., Wiegel, C., Haselmann, U., Meissner, M., Stelzer, E. H.K., and Reynaud, E. G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cytotechnology
Publisher:Springer
ISSN:0920-9069
ISSN (Online):1573-0778
Published Online:04 February 2016

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