Kane, M., Zang, T. M., Rihn, S. J. , Zhang, F., Kueck, T., Alim, M., Schoggins, J., Rice, C. M., Wilson, S. J. and Bieniasz, P. D. (2016) Identification of interferon-stimulated genes with antiretroviral activity. Cell Host and Microbe, 20(3), pp. 392-405. (doi: 10.1016/j.chom.2016.08.005) (PMID:27631702) (PMCID:PMC5026698)
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Abstract
Interferons (IFNs) exert their anti-viral effects by inducing the expression of hundreds of IFN-stimulated genes (ISGs). The activity of known ISGs is insufficient to account for the antiretroviral effects of IFN, suggesting that ISGs with antiretroviral activity are yet to be described. We constructed an arrayed library of ISGs from rhesus macaques and tested the ability of hundreds of individual macaque and human ISGs to inhibit early and late replication steps for 11 members of the retroviridae from various host species. These screens uncovered numerous ISGs with antiretroviral activity at both the early and late stages of virus replication. Detailed analyses of two antiretroviral ISGs indicate that indoleamine 2,3-dioxygenase 1 (IDO1) can inhibit retroviral replication by metabolite depletion while tripartite motif-56 (TRIM56) accentuates ISG induction by IFNα and inhibits the expression of late HIV-1 genes. Overall, these studies reveal numerous host proteins that mediate the antiretroviral activity of IFNs.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by grants from the NIH R3764003 (to P.D.B.), 1F32AI116263-01 (to M.K.), AI091707 (to J.S. and C.M.R.), and UK MRC MR/K024752/1 (to S.J.W.). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Rihn, Dr Suzannah and Wilson, Professor Sam |
| Authors: | Kane, M., Zang, T. M., Rihn, S. J., Zhang, F., Kueck, T., Alim, M., Schoggins, J., Rice, C. M., Wilson, S. J., and Bieniasz, P. D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Cell Host and Microbe |
| Publisher: | Elsevier (Cell Press) |
| ISSN: | 1931-3128 |
| ISSN (Online): | 1934-6069 |
| Published Online: | 14 September 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Cell Host and Microbe 20(3): 392-405 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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