Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a Highly controllable substrate with potential cellular applications

Laing, S., Suriano, R., Lamprou, D. A., Smith, C.-A. , Dalby, M. J. , Mabbott, S., Faulds, K. and Graham, D. (2016) Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a Highly controllable substrate with potential cellular applications. ACS Applied Materials and Interfaces, 8(37), pp. 24844-24852. (doi:10.1021/acsami.6b03860) (PMID:27572916)

Laing, S., Suriano, R., Lamprou, D. A., Smith, C.-A. , Dalby, M. J. , Mabbott, S., Faulds, K. and Graham, D. (2016) Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a Highly controllable substrate with potential cellular applications. ACS Applied Materials and Interfaces, 8(37), pp. 24844-24852. (doi:10.1021/acsami.6b03860) (PMID:27572916)

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Abstract

We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanized silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behavior of polymer arrays which swell/deswell in aqueous solution in response to a change in temperature was successfully characterized by atomic force microscopy (AFM) and Raman spectroscopy: a thermally induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behavior when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and Smith, Mrs Carol-Anne
Authors: Laing, S., Suriano, R., Lamprou, D. A., Smith, C.-A., Dalby, M. J., Mabbott, S., Faulds, K., and Graham, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:ACS Applied Materials and Interfaces
Publisher:American Chemical Society
ISSN:1944-8244
ISSN (Online):1944-8252
Published Online:30 August 2016
Copyright Holders:Copyright © 2016 American Chemical Society
First Published:First published in ACS Applied Materials and Interfaces 8(37):24844-24852
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
608751Dynamic surfaces to mimic mesenchymal stem cell niche functionsMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006908/1RI MOLECULAR CELL & SYSTEMS BIOLOGY