Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidumreaction centers on silver island films

Maćkowski, S., Czechowski, N., Ashraf, K. U., Szalkowski, M., Lokstein, H., Cogdell, R. J. and Kowalska, D. (2016) Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidumreaction centers on silver island films. FEBS Letters, 590(16), pp. 2558-2565. (doi:10.1002/1873-3468.12292) (PMID:27406896)

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Abstract

We focus on the spectral dependence of plasmon-induced enhancement of fluorescence of Chlorobaculum tepidum reaction centers. When deposited on silver island film, they exhibit up to a 60-fold increase in fluorescence. The dependence of enhancement factors on the excitation wavelength is not correlated with the absorption spectrum of the plasmonic structure. In particular, the presence of one (or multiple) trimers of the Fenna–Matthews–Olson (FMO) protein reveals itself in bimodal distribution of enhancement factors for the excitation at 589 nm, the wavelength corresponding to bacteriochlorophyll absorption of FMO and the core of the RC. We conclude that the structure of multichromophoric complexes can substantially affect the impact of plasmonic excitations, which is important in the context of assembling functional biohybrid systems.

Item Type:Articles (Letter)
Additional Information:Research in Poland was supported by theWELCOME/2008/2 project “Hybrid Nanostructuresas a Stepping Stone towards Efficient Artificial Photo-synthesis” funded by the Foundation for PolishScience, DEC-2013/11/B/ST3/03984 and DEC-2013/10/E/ST3/00034 projects from the National ScienceCenter of Poland, and the EuroCores project “BOLD-CATS” funded by the European Science Foundation.Part of the sample characterization was carried out atthe facilities of the National Laboratory FAMO at theInstitute of Physics, NCU in Torun. R.J.C. and H.L.gratefully acknowledge financial support by theBBSRC/EuroCores and the BMBF (Biotechologie2020+, H.L.). K.U.A. is grateful to the University ofGlasgow for financial support.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lokstein, Dr Rer Nat Heiko and Cogdell, Professor Richard and Ashraf, Mr Khuram
Authors: Maćkowski, S., Czechowski, N., Ashraf, K. U., Szalkowski, M., Lokstein, H., Cogdell, R. J., and Kowalska, D.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:FEBS Letters
Publisher:Wiley
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:13 July 2016

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