Yersinia ruckeri isolates recovered from diseased Atlantic Salmon (Salmo salar) in Scotland are more diverse than those from Rainbow Trout (Oncorhynchus mykiss) and represent distinct subpopulations

Ormsby, M. J., Caws, T., Burchmore, R., Wallis, T., Verner-Jeffreys, D. W. and Davies, R. L. (2016) Yersinia ruckeri isolates recovered from diseased Atlantic Salmon (Salmo salar) in Scotland are more diverse than those from Rainbow Trout (Oncorhynchus mykiss) and represent distinct subpopulations. Applied and Environmental Microbiology, 82(19), pp. 5785-5794. (doi:10.1128/aem.01173-16) (PMID:27451448)

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Abstract

Yersinia ruckeri is the etiological agent of enteric redmouth (ERM) disease of farmed salmonids. Enteric redmouth disease is traditionally associated with rainbow trout (Oncorhynchus mykiss, Walbaum), but its incidence in Atlantic salmon (Salmo salar) is increasing. Yersinia ruckeri isolates recovered from diseased Atlantic salmon have been poorly characterized, and very little is known about the relationship of the isolates associated with these two species. Phenotypic approaches were used to characterize 109 Y. ruckeri isolates recovered over a 14-year period from infected Atlantic salmon in Scotland; 26 isolates from infected rainbow trout were also characterized. Biotyping, serotyping, and comparison of outer membrane protein profiles identified 19 Y. ruckeri clones associated with Atlantic salmon but only five associated with rainbow trout; none of the Atlantic salmon clones occurred in rainbow trout and vice versa. These findings suggest that distinct subpopulations of Y. ruckeri are associated with each species. A new O serotype (designated O8) was identified in 56 biotype 1 Atlantic salmon isolates and was the most common serotype identified from 2006 to 2011 and in 2014, suggesting an increased prevalence during the time period sampled. Rainbow trout isolates were represented almost exclusively by the same biotype 2, serotype O1 clone that has been responsible for the majority of ERM outbreaks in this species within the United Kingdom since the 1980s. However, the identification of two biotype 2, serotype O8 isolates in rainbow trout suggests that vaccines containing serotypes O1 and O8 should be evaluated in both rainbow trout and Atlantic salmon for application in Scotland.

Item Type:Articles
Additional Information:This work, including the efforts of Michael J. Ormsby, was funded by an Industrial CASE PhD studentship award from the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/I01554X/1). Additional financial support was provided by the Centre for Environment, Fisheries and Aquaculture Science (Cefas), Ridgeway Biologicals Ltd., Marine Harvest Scotland, and Dawnfresh Seafoods.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and Davies, Dr Robert and Ormsby, Mr Michael
Authors: Ormsby, M. J., Caws, T., Burchmore, R., Wallis, T., Verner-Jeffreys, D. W., and Davies, R. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Applied and Environmental Microbiology
Publisher:American Society for Microbiology
ISSN:0099-2240
ISSN (Online):1098-5336
Published Online:22 July 2016
Copyright Holders:Copyright © 2016 American Society for Microbiology
First Published:First published in Applied and Environmental Microbiology 82(19): 5785-5794
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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