Song, Y., Yin, H. and Huang, W. E. (2016) Raman activated cell sorting. Current Opinion in Chemical Biology, 33, pp. 1-8. (doi: 10.1016/j.cbpa.2016.04.002) (PMID:27100046)
|
Text
130401.pdf - Accepted Version 1MB |
Abstract
Single cell Raman spectra (SCRS) are intrinsic biochemical profiles and ‘chemical images’ of single cells which can be used to characterise phenotypic changes, physiological states and functions of cells. On the base of SCRS, Raman activated cell sorting (RACS) provides a label-free cell sorting approach, which can link single cells to their chemical or phenotypic profiles. Overcoming naturally weak Raman signals, establishing Raman biomarker as sorting criteria to RACS and improving specific sorting technology are three challenges of developing RACS. Advances on Raman spectroscopy such as stimulated Raman scattering (SRS) and pre-screening helped to increase RACS sorting speed. Entire SCRS can be characterised using pattern recognition methods, and specific Raman bands can be extracted as biomarkers for RACS. Recent advances on cell sorting technologies based on microfluidic device and surface-ejection enable accurate and reliable single cell sorting from complex samples. A high throughput RACS will be achievable in near future by integrating fast Raman detection system such as SRS with microfluidic RACS and Raman activated cell ejection (RACE).
| Item Type: | Articles |
|---|---|
| Additional Information: | The authors acknowledge support from EPSRC (EP/M002403/1), NERC (NE/ M002934/1) in the UK. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing |
| Authors: | Song, Y., Yin, H., and Huang, W. E. |
| College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Current Opinion in Chemical Biology |
| Publisher: | Elsevier |
| ISSN: | 1367-5931 |
| Published Online: | 18 April 2016 |
| Copyright Holders: | Copyright © 2016 Elsevier Ltd. |
| First Published: | First published in Current Opinion in Chemical Biology 33: 1-8 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
| Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details