Tomato Juice consumption modifies the urinary peptide profile in Sprague-Dawley rats with induced hepatic steatosis

Martin-Pozuelo, G., Gonzalez-Barrio, R., Barberá, G. G., Albalat, A., García-Alonso, J., Mullen, W. , Mischak, H. and Periag, M. J. (2016) Tomato Juice consumption modifies the urinary peptide profile in Sprague-Dawley rats with induced hepatic steatosis. International Journal of Molecular Sciences, 17(11), 1789. (doi: 10.3390/ijms17111789)

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Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in Western countries, with a high prevalence, and has been shown to increase the risk of type 2 diabetes, cardiovascular disease (CVD), etc. Tomato products contain several natural antioxidants, including lycopene—which has displayed a preventive effect on the development of steatosis and CVD. Accordingly, the aim of the present work was to evaluate the effect of tomato juice consumption on the urinary peptide profile in rats with NAFLD induced by an atherogenic diet and to identify potential peptide biomarkers for diagnosis. Urine samples, collected weekly for four weeks, were analyzed by capillary electrophoresis (CE) coupled to a mass spectrometer (MS). A partial least squares-discriminant analysis (PLS-DA) was carried out to explore the association between differential peptides and treatments. Among the 888 peptides initially identified, a total of 55 were obtained as potential biomarkers. Rats with steatosis after tomato juice intake showed a profile intermediate between that of healthy rats and that of rats with induced hepatic steatosis. Accordingly, tomato products could be considered as a dietary strategy for the impairment of NAFLD, although further research should be carried out to develop a specific biomarkers panel for NAFLD.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Albalat, Dr Amaya and Mullen, Dr Bill and Mischak, Professor Harald and Gonzalez-Barrio, Dr Rocio
Authors: Martin-Pozuelo, G., Gonzalez-Barrio, R., Barberá, G. G., Albalat, A., García-Alonso, J., Mullen, W., Mischak, H., and Periag, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:International Journal of Molecular Sciences
Publisher:MDPI
ISSN:1661-6596
ISSN (Online):1422-0067
Published Online:26 October 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in International Journal of Molecular Sciences 17(11):1789
Publisher Policy:Reproduced under a Creative Commons License

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