Roles of the Nfu Fe–S targeting factors in the trypanosome mitochondrion

Benz, C., Kovarova, J., Králová-Hromadová, I., Pierik, A. J. and Lukeš, J. (2016) Roles of the Nfu Fe–S targeting factors in the trypanosome mitochondrion. International Journal for Parasitology, 46(10), pp. 641-651. (doi:10.1016/j.ijpara.2016.04.006) (PMID:27181928)

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Abstract

Iron–sulphur clusters (ISCs) are protein co-factors essential for a wide range of cellular functions. The core iron–sulphur cluster assembly machinery resides in the mitochondrion, yet due to export of an essential precursor from the organelle, it is also needed for cytosolic and nuclear iron–sulphur cluster assembly. In mitochondria all [4Fe–4S] iron–sulphur clusters are synthesised and transferred to specific apoproteins by so-called iron–sulphur cluster targeting factors. One of these factors is the universally present mitochondrial Nfu1, which in humans is required for the proper assembly of a subset of mitochondrial [4Fe–4S] proteins. Although most eukaryotes harbour a single Nfu1, the genomes of Trypanosoma brucei and related flagellates encode three Nfu genes. All three Nfu proteins localise to the mitochondrion in the procyclic form of T. brucei, and TbNfu2 and TbNfu3 are both individually essential for growth in bloodstream and procyclic forms, suggesting highly specific functions for each of these proteins in the trypanosome cell. Moreover, these two proteins are functional in the iron–sulphur cluster assembly in a heterologous system and rescue the growth defect of a yeast deletion mutant.

Item Type:Articles
Additional Information:This work was supported by the Czech Grant Agency 16-18699S, and a Bioglobe (European Union) grant CZ.1.07/2.3.00/30.0006 to CB and JL. We acknowledge the use of research infrastructure that has received funding from the European Union 7th Framework Program (FP7/2007–2013) under agreement no. 316304.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Benz, Dr Corinna and Kovarova, Ms Julie
Authors: Benz, C., Kovarova, J., Králová-Hromadová, I., Pierik, A. J., and Lukeš, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:International Journal for Parasitology
Publisher:Elsevier
ISSN:0020-7519
Published Online:12 May 2016

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