P16INK4a positive cells in human skin are indicative of local elastic fiber morphology, facial wrinkling, and perceived age

Waaijer, M. E.C., Gunn, D. A., Adams, P. D., Pawlikowski, J. S., Griffiths, C. E.M., van Heemst, D., Slagboom, P. E., Westendorp, R. G.J. and Maier, A. B. (2016) P16INK4a positive cells in human skin are indicative of local elastic fiber morphology, facial wrinkling, and perceived age. Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 71(8), pp. 1022-1028. (doi: 10.1093/gerona/glv114) (PMID:26286607)

[img]
Preview
Text
129695.pdf - Accepted Version

198kB

Abstract

Senescent cells are more prevalent in aged human skin compared to young, but evidence that senescent cells are linked to other biomarkers of aging is scarce. We counted cells positive for the tumor suppressor and senescence associated protein p16INK4a in sun-protected upper-inner arm skin biopsies from 178 participants (aged 45–81 years) of the Leiden Longevity Study. Local elastic fiber morphology, facial wrinkles, and perceived facial age were compared to tertiles of p16INK4a counts, while adjusting for chronological age and other potential confounders. The numbers of epidermal and dermal p16INK4a positive cells were significantly associated with age-associated elastic fiber morphologic characteristics, such as longer and a greater number of elastic fibers. The p16INK4a positive epidermal cells (identified as primarily melanocytes) were also significantly associated with more facial wrinkles and a higher perceived age. Participants in the lowest tertile of epidermal p16INK4a counts looked 3 years younger than those in the highest tertile, independently of chronological age and elastic fiber morphology. In conclusion, p16INK4a positive cell numbers in sun-protected human arm skin are indicative of both local elastic fiber morphology and the extent of aging visible in the face.

Item Type:Articles
Additional Information:This study was supported by the Innovation Oriented research Program on Genomics (SenterNovem; IGE01014, IGE5007), the Centre for Medical Systems Biology (CMSB), the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (NGI/NWO 05040202, 050-060- 810 [NCHA]), Unilever, the EU funded Network of Excellence Lifespan (FP6 036894), and the National Institute on Aging (NIA P01 AG031862). Individual coauthors were funded by the Netherlands Genomics Initiative (NCHA 050-060-810 to P.E.S.) and T32HL007751—NIH Training Grant in Mechanisms of Vascular Disease (J.S.P.).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Adams, Professor Peter
Authors: Waaijer, M. E.C., Gunn, D. A., Adams, P. D., Pawlikowski, J. S., Griffiths, C. E.M., van Heemst, D., Slagboom, P. E., Westendorp, R. G.J., and Maier, A. B.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Publisher:Oxford University Press
ISSN:1079-5006
ISSN (Online):1758-535X
Published Online:18 August 2015
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Journals of Gerontology Series A: Biological Sciences and Medical Sciences 71(8): 1022-1028
Publisher Policy:Reproduced in accordance with the publisher copyright policy

University Staff: Request a correction | Enlighten Editors: Update this record