Wolbachia restricts insect specific flavivirus infection in Aedes aegypti cells

Schnettler, E., Sreenu, V. B. , Mottram, T. and McFarlane, M. (2016) Wolbachia restricts insect specific flavivirus infection in Aedes aegypti cells. Journal of General Virology, 97(11), pp. 3024-3029. (doi:10.1099/jgv.0.000617) (PMID:27692043) (PMCID:PMC5120408)

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Abstract

Mosquito-borne viruses are known to cause disease in humans and livestock and are often difficult to control due the lack of specific antivirals and vaccines. The Wolbachia endosymbiont has been widely studied for its ability to restrict positive strand RNA virus infection in mosquitoes, although little is known about the precise antiviral mechanism. In recent years, a variety of insect-specific viruses have been discovered in mosquitoes and an interaction with mosquito-borne viruses have been reported for some of them; however nothing is known about the effect of Wolbachia on insect specific virus infection in mosquitoes. Here, we show that transinfection of the drosophila derived wMelPop Wolbachia strain into Ae. aegypti-derived cells resulted in inhibition and even clearance of the persistent cell fusing agent flavivirus infection in these cells. This, broadens the antiviral activity of Wolbachia from acute infections to persistent infections and from arboviruses to mosquito-specific viruses. In contrast, no effect on the Phasi-Charoen-like bunyavirus persistent infection in these cells was observed, suggesting a difference in Wolbachia inhibition between positive and negative strand RNA viruses.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Schnettler, Dr Esther and Mottram, Mr Timothy and Vattipally, Dr Sreenu and Mcdonald, Dr Melanie
Authors: Schnettler, E., Sreenu, V. B., Mottram, T., and McFarlane, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Microbiology Society
ISSN:0022-1317
ISSN (Online):1465-2099
Published Online:10 November 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Journal of General Virology 97(11): 3024-3029
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
635361Molecular analyses of arbovirus-host interactionsRichard ElliottWellcome Trust (WELLCOME)099220/B/12/ZMVLS III - CENTRE FOR VIRUS RESEARCH
656341Virus-host interactions in hepatitis C virus infectionJohn McLauchlanMedical Research Council (MRC)MC_UU_12014/1MVLS III - CENTRE FOR VIRUS RESEARCH