A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection

Bayes, H.K., Ritchie, N., Irvine, S. and Evans, T.J. (2016) A murine model of early Pseudomonas aeruginosa lung disease with transition to chronic infection. Scientific Reports, 6, 35838. (doi: 10.1038/srep35838) (PMID:27804985) (PMCID:PMC5090221)

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Abstract

Pseudomonas aeruginosa (PA) remains an important pathogen in patients with cystic fibrosis (CF) lung disease as well as non-CF bronchiectasis and chronic obstructive airways disease. Initial infections are cleared but chronic infection with mucoid strains ensues in the majority of CF patients and specific interventions to prevent this critical infection transition are lacking. The PA bead model has been widely used to study pulmonary P.aeruginosa infection but has limitations in animal husbandry and in accurately mimicking human disease. We have developed an adapted agar bead murine model using a clinical mucoid strain that demonstrates the key features of transition from transitory to chronic airways infection. Infected animals show very limited acute morbidity and mortality, but undergo infection-related weight loss and neutrophilic inflammation, development of anti-pseudomonal antibodies, variable bacterial clearance, endobronchial infection and microbial adaptation with PA small colony variants. We anticipate this model will allow research into the host and microbial factors governing this critical period in Pseudomonas aeruginosa pulmonary pathogenesis when transition to chronicity is occurring.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ritchie, Dr Neil and Bayes, Dr Hannah and Evans, Professor Tom and Irvine, Dr Sharon
Authors: Bayes, H.K., Ritchie, N., Irvine, S., and Evans, T.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Published Online:02 November 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Reports 6:35838
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
558682The Proinflammatory Th17 Response as a Therapeutic Target in Cystic Fibrosis Lung DiseaseTom EvansWellcome Trust (WELLCOME)094779/Z/12AIII - BACTERIOLOGY