Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects: a stable isotope study

Boets, E. et al. (2017) Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects: a stable isotope study. Journal of Physiology, 595(2), pp. 541-555. (doi:10.1113/JP272613) (PMID:27510655)

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Abstract

The short-chain fatty acids (SCFAs), acetate, propionate and butyrate, are bacterial metabolites that mediate the interaction between the diet, the microbiota and the host. In the present study, the systemic availability of SCFAs and their incorporation into biologically relevant molecules was quantified. Known amounts of 13C-labelled acetate, propionate and butyrate were introduced in the colon of 12 healthy subjects using colon delivery capsules and plasma levels of 13C-SCFAs 13C-glucose, 13C-cholesterol and 13C-fatty acids were measured. The butyrate-producing capacity of the intestinal microbiota was also quantified. Systemic availability of colonic-administered acetate, propionate and butyrate was 36%, 9% and 2%, respectively. Conversion of acetate into butyrate (24%) was the most prevalent interconversion by the colonic microbiota and was not related to the butyrate-producing capacity in the faecal samples. Less than 1% of administered acetate was incorporated into cholesterol and <15% in fatty acids. On average, 6% of colonic propionate was incorporated into glucose. The SCFAs were mainly excreted via the lungs after oxidation to 13CO2, whereas less than 0.05% of the SCFAs were excreted into urine. These results will allow future evaluation and quantification of SCFA production from 13C-labelled fibres in the human colon by measurement of 13C-labelled SCFA concentrations in blood.

Item Type:Articles
Additional Information:The work was conducted in the framework of the W. K. KelloggChair in Cereal Science and Nutrition at the KU Leuven (chairholders J. A. Delcour and K. Verbeke) and was further supportedby the Fund for Scientific Research-Flanders (FWO Vlaanderen,Belgium; grants FWO-G.0109.07 and FWO-1.5.131.10).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Preston, Professor Thomas
Authors: Boets, E., Gomand, S. V., Deroover, L., Preston, T., Vermeulen, K., De Preter, V., Hamer, H. M., Van den Mooter, G., De Vuyst, L., Courtin, C. M., Annaert, P., Delcour, J. A., and Verbeke, K. A.
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Journal of Physiology
Publisher:Wiley
ISSN:0022-3751
ISSN (Online):1469-7793
Published Online:18 September 2016
Copyright Holders:Copyright © 2016 Wiley
First Published:First published in Journal of Physiology 595(2):541-555
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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