Husi, H. and Walkinshaw, M. D. (2001) Purification of factor X by hydrophobic interaction chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 755(1-2), pp. 367-371. (doi: 10.1016/S0378-4347(01)00112-8) (PMID:11393727)
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Abstract
Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2-3 M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available.
| Item Type: | Articles (Other) |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Husi, Dr Holger |
| Authors: | Husi, H., and Walkinshaw, M. D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Journal of Chromatography B: Biomedical Sciences and Applications |
| Publisher: | Elsevier |
| ISSN: | 1387-2273 |
| Published Online: | 12 April 2001 |
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