Validation and determination of a reference interval for Canine HbA1c using an immunoturbidimetric assay

Goemans, A. F., Spence, S. J. and Ramsey, I. K. (2017) Validation and determination of a reference interval for Canine HbA1c using an immunoturbidimetric assay. Veterinary Clinical Pathology, 46(2), pp. 227-237. (doi:10.1111/vcp.12477) (PMID:28387422)

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Abstract

Background: Hemoglobin A1c (HbA1c) provides a reliable measure of glycemic control over 2–3 months in human diabetes mellitus. In dogs, presence of HbA1c has been demonstrated, but there are no validated commercial assays. Objective: The purpose of the study was to validate a commercially available automated immunoturbidimetric assay for canine HbA1c and determine an RI in a hospital population. Methods: The specificity of the assay was assessed by inducing glycosylation in vitro using isolated canine hemoglobin, repeatability by measuring canine samples 5 times in succession, long term inter-assay imprecision by measuring supplied control materials, stability using samples stored at 4°C over 5 days and −20°C over 8 weeks, linearity by mixing samples of known HbA1c in differing proportions, and the effect of anticoagulants with paired samples. An RI was determined using EDTA-anticoagulated blood samples from 60 nondiabetic hospitalized animals of various ages and breeds. Hemoglobin A1c was also measured in 10 diabetic dogs. Results: The concentration of HbA1c increased proportionally with glucose concentration in vitro. For repeat measurements, the CV was 4.08% (range 1.16–6.10%). Samples were stable for 5 days at 4°C. The assay was linear within the assessed range. Heparin- and EDTA-anticoagulated blood provided comparable results. The RI for HbA1c was 9–18.5 mmol/mol. There was no apparent effect of age or breed on HbA1c. In diabetic dogs, HbA1c ranged from 14 to 48 mmol/mol. Conclusions: The assay provides a reliable method for canine HbA1c measurement with good analytic performance.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramsey, Professor Ian
Authors: Goemans, A. F., Spence, S. J., and Ramsey, I. K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Veterinary Clinical Pathology
Publisher:Wiley
ISSN:0275-6382
ISSN (Online):1939-165X
Published Online:07 April 2017
Copyright Holders:Copyright © 2017 American Society for Veterinary Clinical Pathology
First Published:First published in Veterinary Clinical Pathology 46(2): 227-237
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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