Lewis, E. E. L., Wheadon, H. , Lewis, N., Yang, J., Mullin, M., Hursthouse, A., Stirling, D., Dalby, M. J. and Berry, C. C. (2016) A quiescent, regeneration-responsive tissue engineered mesenchymal stem cell bone marrow niche model via magnetic levitation. ACS Nano, 10(9), pp. 8346-8354. (doi: 10.1021/acsnano.6b02841) (PMID:27602872)
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Abstract
The bone marrow niche represents a specialized environment that regulates mesenchymal stem cell quiescence and self-renewal, yet fosters stem cell migration and differentiation upon demand. An in vitro model that embodies these features would open up the ability to perform detailed study of stem cell behavior. In this paper we present a simple bone marrow-like niche model, which comprises of nanomagnetically levitated stem cells cultured as multicellular spheroids within a type I collagen gel. The stem cells maintained are nestin positive and remain quiescent until regenerative demand is placed upon them. In response to coculture wounding, they migrate and appropriately differentiate upon engraftment. This tissue engineered regeneration-responsive bone marrow-like niche model will allow for greater understanding of stem cell response to injury and also facilitate as a testing platform for drug candidates in a multiwell plate format.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Lewis, Natasha and Berry, Dr Catherine and Dalby, Professor Matthew and Wheadon, Professor Helen and Yang, Dr Jingli and Mullin, Mrs Margaret |
| Authors: | Lewis, E. E. L., Wheadon, H., Lewis, N., Yang, J., Mullin, M., Hursthouse, A., Stirling, D., Dalby, M. J., and Berry, C. C. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | ACS Nano |
| Publisher: | American Chemical Society |
| ISSN: | 1936-0851 |
| ISSN (Online): | 1936-086X |
| Published Online: | 07 September 2016 |
| Copyright Holders: | Copyright © 2016 American Chemical Society |
| First Published: | First published in ACS Nano 10(9): 8346-8354 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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