A quiescent, regeneration-responsive tissue engineered mesenchymal stem cell bone marrow niche model via magnetic levitation

Lewis, E. E. L., Wheadon, H. , Lewis, N., Yang, J., Mullin, M., Hursthouse, A., Stirling, D., Dalby, M. J. and Berry, C. C. (2016) A quiescent, regeneration-responsive tissue engineered mesenchymal stem cell bone marrow niche model via magnetic levitation. ACS Nano, 10(9), pp. 8346-8354. (doi:10.1021/acsnano.6b02841) (PMID:27602872)

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Abstract

The bone marrow niche represents a specialized environment that regulates mesenchymal stem cell quiescence and self-renewal, yet fosters stem cell migration and differentiation upon demand. An in vitro model that embodies these features would open up the ability to perform detailed study of stem cell behavior. In this paper we present a simple bone marrow-like niche model, which comprises of nanomagnetically levitated stem cells cultured as multicellular spheroids within a type I collagen gel. The stem cells maintained are nestin positive and remain quiescent until regenerative demand is placed upon them. In response to coculture wounding, they migrate and appropriately differentiate upon engraftment. This tissue engineered regeneration-responsive bone marrow-like niche model will allow for greater understanding of stem cell response to injury and also facilitate as a testing platform for drug candidates in a multiwell plate format.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lewis, Natasha and Berry, Dr Catherine and Dalby, Professor Matthew and Yang, Dr Jingli and Mullin, Mrs Margaret and Wheadon, Dr Helen
Authors: Lewis, E. E. L., Wheadon, H., Lewis, N., Yang, J., Mullin, M., Hursthouse, A., Stirling, D., Dalby, M. J., and Berry, C. C.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN:1936-0851
ISSN (Online):1936-086X
Published Online:07 September 2016
Copyright Holders:Copyright © 2016 American Chemical Society
First Published:First published in ACS Nano 10(9): 8346-8354
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
619691Nanoparticles and nanotopography: a nano-toolbox to control stem cell self-renewal via microRNAsCatherine BerryBiotechnology and Biological Sciences Research Council (BBSRC)BB/L008661/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
470563DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING