Identification of Theileria lestoquardi antigens recognized by CD8+ T cells

Goh, S. et al. (2016) Identification of Theileria lestoquardi antigens recognized by CD8+ T cells. PLoS ONE, 11(9), e0162571. (doi: 10.1371/journal.pone.0162571) (PMID:27611868) (PMCID:PMC5017765)

Abstract

As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Weir, Professor Willie and Shiels, Professor Brian
• Authors: Goh, S., Ngugi, D., Lizundia, R., Hostettler, I., Woods, K., Ballingall, K., MacHugh, N. D., Morrison, W. I., Weir, W., Shiels, B., and Werling, D.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: PLoS ONE
• Publisher: Public Library of Science
• ISSN: 1932-6203
• ISSN (Online): 1932-6203
• Copyright Holders: Copyright © 2016 Goh et al.
• First Published: First published in PLoS ONE 11(9):e0162571
• Publisher Policy: Reproduced under a Creative Commons License