Factors influencing cellular immune responses to feline immunodeficiency virus induced by DNA vaccination

Flynn, J.N., Hosie, M.J. , Rigby, M.A., Mackay, N., Cannon, C.A., Dunsford, T., Neil, J.C. and Jarrett, O. (2000) Factors influencing cellular immune responses to feline immunodeficiency virus induced by DNA vaccination. Vaccine, 18(11-12), pp. 1118-1132. (doi: 10.1016/S0264-410X(99)00375-8) (PMID:10590334)

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Abstract

Virus-specific effector cytotoxic T lymphocytes (CTL) were elicited in the peripheral blood of domestic cats following a single intramuscular inoculation of replication defective feline immunodeficiency virus proviral DNA (FIVΔRT). Higher levels of virus-specific cytolysis were observed in the blood when cats were co-inoculated with feline γ-interferon (IFN) DNA. The responses declined by 12 weeks following the first DNA inoculation and were, with the exception of FIV Gag-specific responses in some cats, refractory to repeated DNA inoculations. Nevertheless, a significant proportion of the cats were protected from challenge with homologous virus. The effects of interval between inoculations, route of DNA delivery, and promoter used to regulate viral gene expression on the induction of virus-specific CTLs were evaluated. The highest levels of virus-specific lysis were recorded following intramuscular co-inoculation of FIVΔRT and γ-IFN DNA, where FIV gene expression was under the control of a cytomegalovirus (CMV) promoter. However, the highest levels of protection were observed using the viral 5′LTR as the promoter. These results suggest that a single intramuscular inoculation of FIVΔRT DNA together with γ-IFN DNA may be sufficient to induce virus-specific CTLs and protection.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Jarrett, Professor James and Neil, Professor James and Cannon, Mrs Cecilia
Authors: Flynn, J.N., Hosie, M.J., Rigby, M.A., Mackay, N., Cannon, C.A., Dunsford, T., Neil, J.C., and Jarrett, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Vaccine
Publisher:Elsevier
ISSN:0264-410X
ISSN (Online):1873-2518
Published Online:06 December 1999

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