Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging

Sinjab, F., Kong, K., Gibson, G. , Varma, S., Williams, H., Padgett, M. and Notingher, I. (2016) Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging. Biomedical Optics Express, 7(8), pp. 2993-3006. (doi: 10.1364/BOE.7.002993) (PMID:27570692) (PMCID:PMC4986808)

Abstract

We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples.

• Item Type: Articles
• Additional Information: Funding: UK Engineering and Physical Sciences Research Council (grant number EP/L025620/1) and the UK Medical Research Council (grant number MP_PC_13072 to the University of Nottingham).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gibson, Dr Graham and Padgett, Professor Miles
• Authors: Sinjab, F., Kong, K., Gibson, G., Varma, S., Williams, H., Padgett, M., and Notingher, I.
• College/School: College of Science and Engineering > School of Physics and Astronomy
• Journal Name: Biomedical Optics Express
• Publisher: Optical Society of America
• ISSN: 2156-7085
• ISSN (Online): 2156-7085
• Published Online: 11 July 2016
• Copyright Holders: Copyright © 2016 The Authors
• First Published: First published in Biomedical Optics Express 7(8): 2993-3006
• Publisher Policy: Reproduced under a Creative Commons License