A recombinant feline immunodeficiency virus envelope fusion protein stimulates peripheral blood lymphocytes from naive cats to proliferate in vitro

Beatty, J. A., Reid, G., Rigby, M. A., Neil, J. C., Jarrett, O. and Browning, M. J. (1992) A recombinant feline immunodeficiency virus envelope fusion protein stimulates peripheral blood lymphocytes from naive cats to proliferate in vitro. Veterinary Immunology and Immunopathology, 35(1-2), pp. 143-153. (doi:10.1016/0165-2427(92)90127-C) (PMID:1337393)

Full text not currently available from Enlighten.

Abstract

A region of feline immunodeficiency virus (FIV)/Glasgow-8 external envelope glycoprotein (env) incorporating the third and fourth variable regions (V3/V4) was cloned, inserted into the pGEX vector and expressed in Escherichia coli to yield milligram quantities of the recombinant polypeptide as a fusion protein with glutathione S-transferase. The fusion protein V3/V4GST was used in lymphocyte proliferation assays, where it consistently caused peripheral blood lymphocytes from naive cats to proliferate in a dose-dependent manner. Other FIV fusion proteins produced under identical conditions (V5GST and p24GST) and glutathione S-transferase alone did not cause proliferation in this system. The monoclonal antibody vpg15, which has been shown to block infection of susceptible cells in vitro, did not decrease the response to V3/V4GST. Human peripheral blood lymphocytes did not proliferate in response to V3/V4GST.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: Beatty, J. A., Reid, G., Rigby, M. A., Neil, J. C., Jarrett, O., and Browning, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Veterinary Immunology and Immunopathology
Publisher:Elsevier
ISSN:0165-2427
ISSN (Online):1873-2534
Published Online:13 November 2002

University Staff: Request a correction | Enlighten Editors: Update this record