Immunodiagnosis of feline immunodeficiency virus infection using recombinant viral p17 and p24

Reid, G., Rigby, M.A., McDonald, M., Hosie, M.J., Neil, J.C. and Jarrett, O. (1991) Immunodiagnosis of feline immunodeficiency virus infection using recombinant viral p17 and p24. AIDS, 5(12), pp. 1477-83. (PMID:1667575)

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Abstract

The coding sequences of p17 and p24 of the Glasgow-8 strain of feline immunodeficiency virus (FIV) were amplified using the polymerase chain reaction and cloned into plasmid vectors. The predicted amino-acid sequences of FIV/Glasgow-8 p17 and p24 were compared with those of the Petaluma and PPR isolates of FIV. As seen with other retroviruses, these gag gene products are highly conserved, indicating that the protein products would be suitable antigens to detect anti-FIV antibodies in an immunoassay. Both p17 and p24 were stably expressed in Escherichia coli as fusion proteins with glutathione S transferase. A pure preparation of each fusion protein was obtained from induced bacterial lysates by affinity chromatography using glutathione-agarose beads. These recombinant proteins were used in an enzyme-linked immunosorbent assay to detect antibodies directed against FIV p17 and p24 in cat sera. This assay allows the identification of seropositive cats following infection with FIV and has greater sensitivity and specificity than a currently available immunodiagnostic test.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: Reid, G., Rigby, M.A., McDonald, M., Hosie, M.J., Neil, J.C., and Jarrett, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:AIDS
Publisher:Lippincott Williams & Wilkins
ISSN:0269-9370
ISSN (Online):1473-5571

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