Conservation of the c-myc coding sequence in transduced feline v-myc genes

Stewart, M. A., Forrest, D., McFarlane, R., Onions, D., Wilkie, N. and Neil, J. C. (1986) Conservation of the c-myc coding sequence in transduced feline v-myc genes. Virology, 154(1), pp. 121-134. (doi: 10.1016/0042-6822(86)90435-6) (PMID:3018999)

Abstract

We have cloned the normal feline c-myc locus and determined the nucleotide sequence of all three exons. The feline c-myc gene shows close homology to other mammalian c-myc genes, particularly human c-myc. The feline and human sequences are colinear within the open reading frame from the putative c-myc product but show insertions and deletions relative to each other outside this domain. We have also analyzed a cloned FeLV provirus, CT4, which contains the host-derived myc gene. In this provirus the v-myc sequences are located at the 3′ end of the pol gene, replacing pol and env sequences. Nucleotide sequence analysis of CT4 shows an open reading frame for a v-myc gene product which may be expressed without fusion to any viral protein sequences. This contrasts with another FeLV v-myc (LC), in which myc and gag sequences were found to be fused. Unlike previously identified avian v-myc genes, the feline v-myc genes contain exon 1-derived sequences, but these have been truncated or internally deleted. The FeLV CT4 v-myc sequence shows very few coding changes relative to c-myc and the FeLV LC v-myc coding sequence is unchanged relative to c-myc apart from fusion to gag. These results are discussed in relation to the mechanism of transduction and activation of myc by FeLV.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Neil, Professor James and Stewart, Dr Monica and Onions, Professor David
• Authors: Stewart, M. A., Forrest, D., McFarlane, R., Onions, D., Wilkie, N., and Neil, J. C.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: Virology
• Publisher: Elsevier
• ISSN: 0042-6822
• ISSN (Online): 1096-0341
• Published Online: 05 February 2004