Fluorescence-excitation and emission spectroscopy on single FMO complexes

Löhner, A., Ashraf, K., Cogdell, R. J. and Köhler, J. (2016) Fluorescence-excitation and emission spectroscopy on single FMO complexes. Scientific Reports, 6, 31875. (doi: 10.1038/srep31875) (PMID:27545197) (PMCID:PMC4992959)

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Abstract

In green-sulfur bacteria sunlight is absorbed by antenna structures termed chlorosomes, and transferred to the RC via the Fenna-Matthews-Olson (FMO) complex. FMO consists of three monomers arranged in C3 symmetry where each monomer accommodates eight Bacteriochlorophyll a (BChl a) molecules. It was the first pigment-protein complex for which the structure has been determined with high resolution and since then this complex has been the subject of numerous studies both experimentally and theoretically. Here we report about fluorescence-excitation spectroscopy as well as emission spectroscopy from individual FMO complexes at low temperatures. The individual FMO complexes are subjected to very fast spectral fluctuations smearing out any possible different information from the ensemble data that were recorded under the same experimental conditions. In other words, on the time scales that are experimentally accessible by single-molecule techniques, the FMO complex exhibits ergodic behaviour.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard and Ashraf, Mr Khuram
Authors: Löhner, A., Ashraf, K., Cogdell, R. J., and Köhler, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Reports 6: 31875
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
502612Photosynthetic Antenna Research Center (PARC)Richard CogdellUS Department of Energy (DEN)DE-SC0001035RI MOLECULAR CELL & SYSTEMS BIOLOGY