The 28 S genomic RNA of avian sarcoma virus PRCII codes for the transformation-specific polyprotein P105

De Lamarter, J. F., Neil, J. C., Ghysdael, J. and Vogt, P. K. (1981) The 28 S genomic RNA of avian sarcoma virus PRCII codes for the transformation-specific polyprotein P105. Virology, 112(2), pp. 757-761. (doi:10.1016/0042-6822(81)90321-4)

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Abstract

We have characterized the genomic RNA of the defective avian sarcoma virus PRCII. Replicating virus which consists of transforming PRCII and a nontransforming but replication-competent helper, PRCII-AV, contains two RNA species. One is identical in size to the 35 S genome of avian leukosis helper viruses. The second component migrates slightly faster than 28 S ribosomal RNA in polyacrylamide gel electrophoresis but co-sediments with this RNA in sucrose gradients. In vitro translation across a gradient of velocity-sedimented poly(A)-containing PRCII virion RNA yielded three major proteins. The virion protein precursors Pr76gag and Pr180gag-pol were translated from 35 S RNA, while the transformation-specific polyprotein P105 was translated from 28 S RNA. P105 may be the only protein coded for by the PRCII genome, although this product would not exhaust the coding capacity of 28 S RNA. Whether translated in vitro or immunoprecipitated from transformed cells, P105 was essentially identical as demonstrated by comparative peptide maps.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: De Lamarter, J. F., Neil, J. C., Ghysdael, J., and Vogt, P. K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
Publisher:Elsevier
ISSN:0042-6822
ISSN (Online):1096-0341
Published Online:09 June 2004

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