The pathogenicity and defectiveness of PRCII: a new type of avian sarcoma virus

Breitman, M. L., Neil, J. C. , Moscovici, C. and Vogt, P. K. (1981) The pathogenicity and defectiveness of PRCII: a new type of avian sarcoma virus. Virology, 108(1), pp. 1-12. (doi: 10.1016/0042-6822(81)90522-5)

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The avian sarcoma virus PRCII (Poultry Research Centre No. 2) transforms fibroblastsin vitro and causes fibromyxosarcomasin vivo. These growths develop after a longer period of latency than Rous sarcomas. PRCII is defective in replication and depends upon an associated helper virus, PRCII AV, for the production of infectious progeny. PRCII AV contains both subgroup A and B envelope determinants. Nonproducer cells transformed in single infection with PRCII do not release viral particles detectable by[3H]uridine incorporation or by reverse transcriptase assay. However, transforming virus can be rescued following super-infection with helper viruses of the chicken leukosis group. Immune precipitation analyses show that PRCII-transformed nonproducer cells do not synthesize the three replicative gene products, Pr76gag, Pr180gag-pol, and gPr95env, but contain a virus-specific protein of 105,000 molecular weight. This PRCII-p105 is precipitable with anti-gag serum, but not with anti-pol serum, anti-env serum, or a broadly reactive anti-src serum. No evidence was found for the expression of p60src in PRCII-transformed cells. PRCII thus represents a new class of avian sarcoma virus distinct from Rous sarcoma virus and its relatives. In the accompanying paper[Neilet al., Virology107, 000 (1980)] we present evidence that PRCII-p105 contains novel sequences not encoded by other transforming oncoviruses.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: Breitman, M. L., Neil, J. C., Moscovici, C., and Vogt, P. K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
ISSN (Online):1096-0341
Published Online:28 June 2006

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