Neil, J. C. , Ghysdael, J. and Vogt, P. K. (1981) Tyrosine-specific protein kinase activity associated with p105 of avian sarcoma virus PRCII. Virology, 109(1), pp. 223-228. (doi: 10.1016/0042-6822(81)90493-1)
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Abstract
A protein kinase activity was found to be associated with the transformation-specific polyprotein (p105) of avian sarcoma virus PRCII. The kinase was detected in immune complexes with antisera reactive with the gag sequences of p105. Addition of [γ-32P]ATP to these complexes resulted in phosphorylation of p105 and, with some sera, phosphorylation of immunoglobulin heavy chain. Phosphoamino acid analysis showed that phosphotyrosine was the major product of the in vitro kinase reaction and the major phosphoamino acid of p105 extracted from 32P-labeled cells. The same tryptic peptides of p105 were found to be labeled by the in vitro kinase reaction and by 32P labeling of PRCII-transformed cells. These phosphopeptides were not found in Pr76gag Thus, like Rous sarcoma virus, PRCII has an associated tyrosine-specific protein kinase which may be responsible for its transforming activity.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Neil, Professor James |
Authors: | Neil, J. C., Ghysdael, J., and Vogt, P. K. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Virology |
Publisher: | Elsevier |
ISSN: | 0042-6822 |
ISSN (Online): | 1096-0341 |
Published Online: | 09 June 2004 |
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