Polypeptides of feline leukemia virus: a glycosylated gag-related protein is released into culture fluids

Neil, J. C. , Smart, J. E., Hayman, M. J. and Jarrett, O. (1980) Polypeptides of feline leukemia virus: a glycosylated gag-related protein is released into culture fluids. Virology, 105(1), pp. 250-253. (doi:10.1016/0042-6822(80)90173-7) (PMID:6251608)

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Abstract

Cells infected with feline leukemia virus (FeLV) shed viral antigens into cell culture fluids. Immunoprecipitation and SDS-PAGE analyses of radiolabeled antigens show that the major virus-specific protein released is a molecule of 40,000 molecular weight which carries gag gene product antigenic determinants. The 40,000 molecular weight (40K) protein was found to be glycosylated and was precipitable with antisera to FeLV gag proteins p27, p12, and pl0 but not p15. Tryptic peptide mapping confirmed the relatedness of FeLV p27 and the 40K glycosylated protein, which was found to be released from all FeLV-infected cells, including untransformed embryonic fibroblasts and transformed lymphoblastoid cells. These results suggest that the FeLV gag gene proteins, like those of murine leukemia viruses, can be processed in two distinct pathways and that this is independent of cell type.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neil, Professor James
Authors: Neil, J. C., Smart, J. E., Hayman, M. J., and Jarrett, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
Publisher:Elsevier
ISSN:0042-6822
ISSN (Online):1096-0341
Published Online:06 February 2004

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