Carpena, N. , Manning, K. A., Dokland, T., Marina, A. and Penadés, J. R. (2016) Convergent evolution of pathogenicity islands in helper cos phage interference. Philosophical Transactions of the Royal Society B: Biological Sciences, 371(1707), 20150505. (doi: 10.1098/rstb.2015.0505) (PMID:27672154) (PMCID:PMC5052747)
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Abstract
Staphylococcus aureus pathogenicity islands (SaPIs) are phage satellites that exploit the life cycle of their helper phages for their own benefit. Most SaPIs are packaged by their helper phages using a headful (pac) packaging mechanism. These SaPIs interfere with pac phage reproduction through a variety of strategies, including the redirection of phage capsid assembly to form small capsids, a process that depends on the expression of the SaPI-encoded cpmA and cpmB genes. Another SaPI subfamily is induced and packaged by cos-type phages, and although these cos SaPIs also block the life cycle of their inducing phages, the basis for this mechanism of interference remains to be deciphered. Here we have identified and characterized one mechanism by which the SaPIs interfere with cos phage reproduction. This mechanism depends on a SaPI-encoded gene, ccm, which encodes a protein involved in the production of small isometric capsids, compared with the prolate helper phage capsids. As the Ccm and CpmAB proteins are completely unrelated in sequence, this strategy represents a fascinating example of convergent evolution. Moreover, this result also indicates that the production of SaPI-sized particles is a widespread strategy of phage interference conserved during SaPI evolution.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Penades, Prof Jose R and Carpena Garcia, Miss Nuria |
| Authors: | Carpena, N., Manning, K. A., Dokland, T., Marina, A., and Penadés, J. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Philosophical Transactions of the Royal Society B: Biological Sciences |
| Publisher: | The Royal Society |
| ISSN: | 0962-8436 |
| ISSN (Online): | 1471-2970 |
| Published Online: | 26 September 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Philosophical Transactions of the Royal Society B: Biological Sciences 371(1707):20150505 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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