Convergent evolution of pathogenicity islands in helper cos phage interference

Carpena, N. , Manning, K. A., Dokland, T., Marina, A. and Penadés, J. R. (2016) Convergent evolution of pathogenicity islands in helper cos phage interference. Philosophical Transactions of the Royal Society B: Biological Sciences, 371(1707), 20150505. (doi:10.1098/rstb.2015.0505) (PMID:27672154) (PMCID:PMC5052747)

[img]
Preview
Text
123582.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

Staphylococcus aureus pathogenicity islands (SaPIs) are phage satellites that exploit the life cycle of their helper phages for their own benefit. Most SaPIs are packaged by their helper phages using a headful (pac) packaging mechanism. These SaPIs interfere with pac phage reproduction through a variety of strategies, including the redirection of phage capsid assembly to form small capsids, a process that depends on the expression of the SaPI-encoded cpmA and cpmB genes. Another SaPI subfamily is induced and packaged by cos-type phages, and although these cos SaPIs also block the life cycle of their inducing phages, the basis for this mechanism of interference remains to be deciphered. Here we have identified and characterized one mechanism by which the SaPIs interfere with cos phage reproduction. This mechanism depends on a SaPI-encoded gene, ccm, which encodes a protein involved in the production of small isometric capsids, compared with the prolate helper phage capsids. As the Ccm and CpmAB proteins are completely unrelated in sequence, this strategy represents a fascinating example of convergent evolution. Moreover, this result also indicates that the production of SaPI-sized particles is a widespread strategy of phage interference conserved during SaPI evolution.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Penades, Professor Jose R and Carpena Garcia, Miss Nuria
Authors: Carpena, N., Manning, K. A., Dokland, T., Marina, A., and Penadés, J. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Philosophical Transactions of the Royal Society B: Biological Sciences
Publisher:The Royal Society
ISSN:0962-8436
ISSN (Online):1471-2970
Published Online:26 September 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Philosophical Transactions of the Royal Society B: Biological Sciences 371(1707):20150505
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
693781Understanding a novel mechanim involving pathogenity islands in the transfer of unlinked chromosomal virulence genesJose R PenadesBiotechnology and Biological Sciences Research Council (BBSRC)BB/N002873/1RI INFECTION IMMUNITY & INFLAMMATION
660772Molecular biology of the PICIs, a novel and widespread family of mobile genetic elements involved in bacterial virulenceJose R PenadesMedical Research Council (MRC)MR/M003876/1RI INFECTION IMMUNITY & INFLAMMATION