Deletion of AMPKα1 attenuates the anticontractile effect of perivascular adipose tissue (PVAT) and reduces adiponectin release

Almabrouk, T. A. M. , Ugusman, A. B., Katwan, O. J., Salt, I. P. and Kennedy, S. (2017) Deletion of AMPKα1 attenuates the anticontractile effect of perivascular adipose tissue (PVAT) and reduces adiponectin release. British Journal of Pharmacology, 174(20), pp. 3398-3410. (doi:10.1111/bph.13633) (PMID:27668984)

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Abstract

Background and Purpose: Perivascular adipose tissue (PVAT) surrounds most blood vessels and secretes numerous active substances, including adiponectin, which produce a net anticontractile effect in healthy individuals. AMPK is a key mediator of cellular energy balance and may mediate the vascular effects of adiponectin. In this study, we investigated the role of AMPK within PVAT in mediating the anticontractile effect of PVAT. Experimental Approach: Endothelium-denuded aortic rings from wild-type (WT; Sv129) and α1AMPK knockout (KO) mice were mounted on a wire myograph. Dose–response curves to the AMPK-independent vasodilator cromakalim were studied in vessels with and without PVAT, and effect of pre-incubation with conditioned media and adiponectin on relaxation was also studied. The effect of AMPKα1 KO on the secretory profile of PVAT was assessed by elisa. Key Results: Thoracic aortic PVAT from KO mice was morphologically indistinct from that of WT and primarily composed of brown adipose tissue. PVAT augmented relaxation to cromakalim in WT but not KO aortic rings. Addition of WT PVAT augmented relaxation in KO aortic rings but KO PVAT had no effect in WT rings. PVAT from KO mice secreted significantly less adiponectin and addition of adiponectin to either KO or WT aortic rings without PVAT augmented relaxation to cromakalim. An adiponectin blocking peptide significantly attenuated relaxation in WT rings with PVAT but not in KO rings. Conclusions and Implications: AMPKα1 has a critical role in maintaining the anticontractile actions of PVAT; an effect independent of the endothelium but likely mediated through altered adiponectin secretion or sensitivity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salt, Dr Ian and ALMABROUK, Tarek Ali Mohamed and Kennedy, Dr Simon
Authors: Almabrouk, T. A. M., Ugusman, A. B., Katwan, O. J., Salt, I. P., and Kennedy, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:British Journal of Pharmacology
Publisher:Wiley
ISSN:0007-1188
ISSN (Online):1476-5381
Published Online:26 September 2016
Copyright Holders:Copyright © 2016 The British Pharmacological Society
First Published:First published in British Journal of Pharmacology 174(20):3398-3410
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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