UAP56 is a conserved crucial component of a divergent mRNA export pathway in Toxoplasma gondii

Serpeloni, M., Jiménez-Ruiz, E. , Vidal, N. M., Kroeber, C., Andenmatten, N., Lemgruber, L. , Mörking, P., Pall, G. S., Meisner, M. and Ávila, A. R. (2016) UAP56 is a conserved crucial component of a divergent mRNA export pathway in Toxoplasma gondii. Molecular Microbiology, 102(4), pp. 672-689. (doi: 10.1111/mmi.13485) (PMID:27542978) (PMCID:PMC5118106)

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Abstract

Nucleo-cytoplasmic RNA export is an essential post-transcriptional step to control gene expression in eukaryotic cells and is poorly understood in apicomplexan parasites. With the exception of UAP56, a component of TREX (Transcription Export) complex, other components of mRNA export machinery are not well conserved in divergent supergroups. Here, we use Toxoplasma gondii as a model system to functionally characterize TgUAP56 and its potential interaction factors. We demonstrate that TgUAP56 is crucial for mRNA export and that functional interference leads to significant accumulation of mRNA in the nucleus. It was necessary to employ bioinformatics and phylogenetic analysis to identify orthologs related to mRNA export, which show a remarkable low level of conservation in T. gondii. We adapted a conditional Cas9/CRISPR system to carry out a genetic screen to verify if these factors were involved in mRNA export in T. gondii. Only the disruption of TgRRM_1330 caused accumulation of mRNA in the nucleus as found with TgUAP56. This protein is potentially a divergent partner of TgUAP56, and provides insight into a divergent mRNA export pathway in apicomplexans.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Avila, Dr Andrea and Pall, Dr Gurman and Serpeloni, Mrs Mariana and Jimenez Ruiz, Dr Elena and Lemgruber Soares, Dr Leandro
Authors: Serpeloni, M., Jiménez-Ruiz, E., Vidal, N. M., Kroeber, C., Andenmatten, N., Lemgruber, L., Mörking, P., Pall, G. S., Meisner, M., and Ávila, A. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular Microbiology
Publisher:Wiley
ISSN:0950-382X
ISSN (Online):1365-2958
Published Online:14 September 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Molecular Microbiology 102(4):672-689
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501601Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOME)087582/Z/08/ZIII - PARASITOLOGY
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY