Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing

Cao, Z. and Bulleid, N. J. (2015) Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. Biophysics Reports, 1(1), pp. 14-17. (doi:10.1007/s41048-015-0007-y) (PMID:26942215) (PMCID:PMC4762124)

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Abstract

The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously. This assay only requires standard equipment and can compare different samples on the same gel. It requires much less time than conventional 2D gels and gives more information than Western blotting with an antibody specific for hyperoxidized peroxiredoxin. This method could also be used to monitor protein modification with a charge difference such as phosphorylation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cao, Dr Zhenbo and Bulleid, Professor Neil
Authors: Cao, Z., and Bulleid, N. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biophysics Reports
Publisher:Springer Berlin Heidelberg
ISSN:2364-3439
ISSN (Online):2364-342
Published Online:25 August 2015
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Biophysics Reports 1(1):14-17
Publisher Policy:Reproduced under a Creative Commons License

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