Enhanced neurochemical profile of the rat brain using in vivo 1H NMR spectroscopy at 16.4 T

Hong, S.-T., Balla, D. Z., Shajan, G., Choi, C., Uğurbil, K. and Pohmann, R. (2011) Enhanced neurochemical profile of the rat brain using in vivo 1H NMR spectroscopy at 16.4 T. Magnetic Resonance in Medicine, 65(1), pp. 28-34. (doi:10.1002/mrm.22609) (PMID:20928884)

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Abstract

Single voxel magnetic resonance spectroscopy with ultrashort echo time was implemented at 16.4 T to enhance the neurochemical profile of the rat brain in vivo. A TE of 1.7 msec was achieved by sequence optimization and by using short-duration asymmetric pulses. Macromolecular signal components were parameterized individually and included in the quantitative analysis, replacing the use of a metabolite-nulled spectrum. Because of the high spectral dispersion, several signals close to the water line could be detected, and adjacent peaks could be resolved. All 20 metabolites detected in this study were quantified with Cramér-Rao lower bounds below 20%, implying reliable quantification accuracy. The signal of acetate was detected for the first time in rat brain in vivo with Cramér-Rao lower bounds of 16% and a concentration of 0.52 μmol/g. The absolute concentrations of most metabolites showed close agreement with values previously measured using in vivo 1H NMR spectroscopy and in vitro biochemical assay.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gunamony, Dr Shajan
Authors: Hong, S.-T., Balla, D. Z., Shajan, G., Choi, C., Uğurbil, K., and Pohmann, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Magnetic Resonance in Medicine
Publisher:Wiley
ISSN:0740-3194
ISSN (Online):1522-2594
Published Online:06 October 2010

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