Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi: 10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)
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Abstract
The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS-protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Quinn, Dr Steven and Magennis, Dr Steven and Morten, Dr Michael |
Authors: | Dalton, C. E., Quinn, S. D., Rafferty, A., Morten, M. J., Gardiner, J. M., and Magennis, S. W. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | ChemPhysChem |
Publisher: | Wiley-VHC Verlag |
ISSN: | 1439-4235 |
ISSN (Online): | 1439-7641 |
Published Online: | 18 August 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in ChemPhysChem 17(21):3442-3446 |
Publisher Policy: | Reproduced under a Creative Commons License |
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