Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide

Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi:10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)

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Abstract

The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS-protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Magennis, Dr Steven and Quinn, Dr Steven and Morten, Dr Michael
Authors: Dalton, C. E., Quinn, S. D., Rafferty, A., Morten, M. J., Gardiner, J. M., and Magennis, S. W.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:ChemPhysChem
Publisher:Wiley-VHC Verlag
ISSN:1439-4235
ISSN (Online):1439-7641
Published Online:18 August 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in ChemPhysChem 17(21):3442-3446
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
647971Speeding and stuttering: analysing the dynamics of DNA replication at the single molecule levelSteven MagennisBiotechnology and Biological Sciences Research Council (BBSRC)BB/K001957/1SCHOOL OF CHEMISTRY
657181From nature to nano: structure, dynamics and reactivity of DNA three-way junctionsSteven MagennisEngineering & Physical Sciences Research Council (EPSRC)EP/L027003/1SCHOOL OF CHEMISTRY