A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species

Thomson, S., Innes, E. A., Jonsson, N. N. and Katzer, F. (2016) A multiplex PCR test to identify four common cattle-adapted Cryptosporidium species. Parasitology Open, 2, e5. (doi:10.1017/pao.2016.2)

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Abstract

Cryptosporidium is a well-known cause of neonatal enteritis in cattle worldwide. Cattle are commonly infected with four different species of Cryptosporidium but only one of these, Cryptosporidium parvum, is associated with clinical disease. Identification of species in cases of calf scour can give an indication if Cryptosporidium is the causative agent or not. In addition, C. parvum is a zoonotic species and so has implications for human health, for this reason it is important to identify the species of Cryptosporidium infecting cattle particularly where a farm is implicated in an outbreak of cryptosporidiosis in humans. Here a multiplex PCR test, which can identify the four common cattle-adapted Cryptosporidium species, including C. parvum, has been developed. This test allows quick and accurate detection of Cryptosporidium species in cattle fecal samples including mixed infections, which could be missed by the more common method of sequencing the same gene.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jonsson, Professor Nicholas and Innes, Professor Elisabeth
Authors: Thomson, S., Innes, E. A., Jonsson, N. N., and Katzer, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Parasitology Open
Publisher:Cambridge University Press
ISSN:2055-7094
ISSN (Online):2055-7094
Published Online:22 April 2016
Copyright Holders:Copyright © 2016 Cambridge University Press
First Published:First published in Parasitology Open 2:e5
Publisher Policy:Reproduced under a Creative Commons License

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