Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Ero1β

Dias-Gunasekara, S., Gubbens, J., van Lith, M., Dunne, C., Williams, J.A.G., Kataky, R., Scoones, D., Lapthorn, A. , Bulleid, N.J. and Benham, A.M. (2005) Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Ero1β. Journal of Biological Chemistry, 280, pp. 33066-33075. (doi:10.1074/jbc.M505023200)

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Abstract

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Understanding disulfide bond catalysis in mammals is important because of the involvement of protein misfolding in conditions such as diabetes, arthritis, cancer, and aging. Mammals express two related Ero proteins, Ero1α and Ero1β. Ero1β is incompletely characterized but is of physiological interest because it is induced by the unfolded protein response. Here, we show that Ero1β can form homodimers and mixed heterodimers with Ero1α, in addition to Ero-PDI dimers. Ero-Ero dimers require the Ero active site, occur in vivo, and can be modeled onto the Ero1p crystal structure. Our data indicate that the Ero1β protein is constitutively strongly expressed in the stomach and the pancreas, but in a cell-specific fashion. In the stomach, selective expression of Ero1β occurs in the enzyme-producing chief cells. In pancreatic islets, Ero1β expression is high, but is inversely correlated with PDI and PDIp levels, demonstrating that cell-specific differences exist in the regulation of oxidative protein folding in vivo.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lapthorn, Dr Adrian and Bulleid, Professor Neil
Authors: Dias-Gunasekara, S., Gubbens, J., van Lith, M., Dunne, C., Williams, J.A.G., Kataky, R., Scoones, D., Lapthorn, A., Bulleid, N.J., and Benham, A.M.
College/School:College of Science and Engineering > School of Chemistry
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Biological Chemistry
Journal Abbr.:J Biol Chem.
Publisher:American Society for Biochemistry and Molecular Biology, Inc.
ISSN:0021-9258
ISSN (Online):1083-351X
Published Online:12 July 2005

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