Jordan, P., Leach, J., Padgett, M. , Blackburn, P., Isaacs, N., Goksor, M., Hanstorp, D., Wright, A., Girkin, J. and Cooper, J. (2005) Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab on a Chip, 5(11), pp. 1224-1228. (doi: 10.1039/B509218C)
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Publisher's URL: http://dx.doi.org/10.1039/B509218C
Abstract
We report the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Wright, Dr Amanda and Padgett, Professor Miles and Cooper, Professor Jonathan and Girkin, Prof John and Leach, Mr Jonathan |
Authors: | Jordan, P., Leach, J., Padgett, M., Blackburn, P., Isaacs, N., Goksor, M., Hanstorp, D., Wright, A., Girkin, J., and Cooper, J. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Physics and Astronomy |
Journal Name: | Lab on a Chip |
Publisher: | Royal Society of Chemistry |
ISSN: | 1473-0197 |
ISSN (Online): | 1473-0189 |
Published Online: | 30 September 2005 |
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