Mao, K., Yang, Z. , Du, P., Xu, Z., Wang, Z. and Li, X. (2016) G-quadruplex–hemin DNAzyme molecular beacon probe for the detection of methamphetamine. RSC Advances, 6(67), pp. 62754-62759. (doi: 10.1039/c6ra04912e)
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Abstract
In this work, a simple, cost-effective, and label-free biosensor was constructed for methamphetamine (METH) detection. The biosensor consists of a G-quadruplex–hemin DNAzyme molecular beacon (DNAzyme MB), a METH aptamer, and a colorimetric substrate. The DNAzyme MB loses peroxidase activity when it hybridizes with the METH aptamer. In the presence of METH, DNAzyme MB dissociates from the inactive hybrid due to preferable hybridization of METH with the aptamer. This process recovers the activity of DNAzyme MB, which catalyzes a reaction with the colorimetric substrate to yield measurable signals. Under optimized conditions, a detection limit as low as 0.5 nM (74.6 ng L−1) was achieved. Common illicit drugs were found to have little interference on detection of METH. Recoveries of METH spiked in urines of addicts were greater than 85%. Good agreement was observed between METH concentrations in urines determined by the sensor and by liquid chromatography-tandem mass spectrometer. These results indicate that the G-quadruplex–hemin DNAzyme MB probe holds promise to detect METH not only in biological samples, but also in environmental matrices.
Item Type: | Articles |
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Additional Information: | We gratefully acknowledge the support from the Natural Science Foundation of China (NSFC) (No. 41371442 and 41401566). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Yang, Dr Zhugen |
Authors: | Mao, K., Yang, Z., Du, P., Xu, Z., Wang, Z., and Li, X. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | RSC Advances |
Publisher: | Royal Society of Chemistry |
ISSN: | 2046-2069 |
ISSN (Online): | 2046-2069 |
Published Online: | 22 June 2016 |
Copyright Holders: | Copyright © 2016 The Royal Society of Chemistry |
First Published: | First published in RSC Advances 2016(6):62754-62759 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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