Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds

Piola, M., Soncini, M., Cantini, M. , Sadr, N., Ferrario, G. and Fiore, G. B. (2013) Design and functional testing of a multichamber perfusion platform for three-dimensional scaffolds. Scientific World Journal, 2013, 123974. (doi:10.1155/2013/123974) (PMID:24453787) (PMCID:PMC3885203)

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Abstract

Perfusion culture systems are widely used in tissue engineering applications for enhancing cell culture viability in the core of three-dimensional scaffolds. In this work, we present a multichamber confined-flow perfusion system, designed to provide a straightforward platform for three-dimensional dynamic cell cultures. The device comprises 6 culture chambers allowing independent and simultaneous experiments in controlled conditions. Each chamber consists of three parts: a housing, a deformable scaffold-holder cartridge, and a 7 mL reservoir, which couples water-tightly with the housing compressing the cartridge. Short-term dynamic cell seeding experiments were carried out with MC3T3-E1 cells seeded into polycaprolactone porous scaffolds. Preliminary results revealed that the application of flow perfusion through the scaffold favored the penetration of the cells to its interior, producing a more homogeneous distribution of cells with respect to dropwise or injection seeding methods. The culture chamber layout was conceived with the aim of simplifying the user operations under laminar flow hood and minimizing the risks for contamination during handling and operation. Furthermore, a compact size, a small number of components, and the use of bayonet couplings ensured a simple, fast, and sterility-promoting assembling. Finally, preliminary in vitro tests proved the efficacy of the system in enhancing cell seeding efficiency, opening the way for further studies addressing long-term scaffold colonization.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cantini, Dr Marco
Authors: Piola, M., Soncini, M., Cantini, M., Sadr, N., Ferrario, G., and Fiore, G. B.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Scientific World Journal
Publisher:Hindawi Publishing Corporation
ISSN:2356-6140
ISSN (Online):1537-744X
Copyright Holders:Copyright © 2013 Marco Piola et al.
First Published:First published in Scientific World Journal123974
Publisher Policy:Reproduced under a creative commons license

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